Figure 1.

Example of the expected thickness of the dissected SEZ tissue

(A) Frontal view of the dissected piece (SEZ ventricle face).

(B) Rear view of the dissected piece (striatum face). Note that, although several striatal white fibers remain attached to the SEZ, they are scarce and it is possible to visualize areas of clean tissue between them.

(C) A partial lateral view showing the expected thickness of the tissue piece.

(D) The same frontal view as in A, showing that the dissected tissue should be thin enough to be translucent.

(E and F) Bright-field images of SEZ homogenates showing expected viable cell (orange arrowheads) to debris ratio in a suboptimal (E) vs. optimal (F) preparation.

Scale bars: A–D, 1 mm; E and F, 50 μm.