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. 2021 Apr 5;2(2):100425. doi: 10.1016/j.xpro.2021.100425

Figure 3.

Figure 3

Comparison between GLAST and GFAP::GFP-based identification of SEZ NSCs and NPCs

(A) GFAP::GFP reporter expression and GLAST immunostaining of SEZ Lin– cells.

(B) GLAST and CD24 expression in the GFAP::GFP+ fraction.

(C) Quantification of PROM1+ cells present in the different SEZ cell types identified (n = 3).

(D) Gating strategy for aNSC and qNSC identification as in Codega et al. (Codega et al., 2014), and quantification of GLAST– GFAP::GFP+ aNSCs (n = 3).

(E) Gating strategy for NPC identification similar to Dulken et al. (Dulken et al., 2017), and distribution of GFAP::GFP– EGFR+ NPCs based on GLAST and PROM1 expression (n = 3).

(F) Representative FACS plot showing the GLAST and CD9 immunostaining of GFAP::GFP-only cells identified in panel D and distribution of GFAP::GFP+ PROM1– astrocytes based in GLAST and CD9 expression (n = 3).