| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD45-BUV421 (clone, rat 30-F11) | BD Biosciences | Cat#563890; RRID: AB_2651151 |
| O4-AF405 (clone, mouse O4) | R&D | Cat#FAB1326V |
| CD31-BUV421 (clone, rat 390) | BD Biosciences | Cat#563356; RRID: AB_2738154 |
| Ter119-BUV421 (clone, rat TER-119) | BD Biosciences | Cat#563998, RRID:AB_2738534 |
| GLAST-PE (clone, mouse ACSA-1) | Miltenyi | Cat#130-095-821; RRID: AB_10829184 |
| O4-Biotin (clone, mouse O4) | Miltenyi | Cat#130-095-895; RRID: AB_10828154 |
| CD24-PerCP-Cy5.5 (clone, rat M1/69) | BD Biosciences | Cat#562360; RRID: AB_11151895 |
| CD24-BB700 (clone, rat M1/69) | BD Biosciences | Cat#746122; RRID: AB_2743489 |
| CD9-Vio770 (clone, rat MZ3) | Miltenyi | Cat#130-102-384; RRID: AB_2659590 |
| CD133-Biotin (clone, rat 13A4) | eBioscience | Cat#13-1331-82; RRID: AB_466591 |
| Chemicals, peptides, and recombinant proteins | ||
| 5-Ethynyl-2′- deoxyuridine (EdU) | Life Technologies | Cat#E10187; CAS: 61135-33-9 |
| 4′, 6-diamidino-2′-phenylindole, dihydrochloride (DAPI) | Sigma | Cat#D9542; CAS: 28718-90-3 |
| BD Cytofix/CytopermTM | BD Biosciences | Cat#554722 |
| HBSS 10× | Biowest | Cat#X0507 |
| EDTA | Sigma | Cat#E6511; CAS: 10378-23-1 |
| HEPES 1M | Biowest | Cat#L0180; CAS: 7365-45-9 |
| D(+)-Glucose | PanReac | Cat#141341; CAS: 50-99-7 |
| BSA | Sigma | Cat#B4287; CAS: 9048-46-8 |
| DMEM/F12 | Gibco | Cat#11320-074 |
| Sodium bicarbonate | Sigma | Cat#S8761-500; CAS: 144-55-8 |
| L-Glutamine | Gibco | Cat#25030024; CAS: 56-85-9 |
| Antibiotic/Antimycotic (100×) | Gibco | Cat#15240062 |
| Dulbecco’s Phosphate Buffered Saline (DPBS) | Sigma | Cat#D1408 |
| Trypsin inhibitor | Sigma | Cat#T6522; CAS: 9035-81-8 |
| Epidermal growth factor (EGF)-Alexa FluorTM 488 | Molecular Probes | Cat#E13345 |
| Streptavidin-BV421 | BD Biosciences | Cat# 563259; RRID: AB_2869475 |
| Critical commercial assays | ||
| Neural Tissue Dissociation kit (T) | Miltenyi | Cat#130-093-231 |
| Click-iTTM Plus EdU Alexa FluorTM 647 Flow Cytometry Assay Kit | Thermo Fisher | Cat#C10634 |
| Dead cell removal kit (optional) | Miltenyi | Cat#130-090-101 |
| Myelin removal kit (optional) | Miltenyi | Cat#130-096-731 |
| Experimental models: organisms/strains | ||
| Mice C57BL/6J (male and female; 2–4 months old) | Jackson Labs. | Cat#000664;RRID: IMSR_JAX:000664 |
| Software and algorithms | ||
| FlowJoTM | Becton Dickinson | RRID:SCR_008520 |
| Deposited data | ||
| Data sets | Belenguer et al., 2021 | GSE138243 |
| Other | ||
| gentleMACSTM Octo Dissociator with Heaters | Miltenyi Biotec | Cat#130-042-109 |
| gentleMACS tubes | Miltenyi | Cat#130-096-334 |
| LSR-Fortessa Cytometera | Becton Dickinson | N/A |
| 15 mL centrifuge tubes | Corning | Cat#CLS430791 |
| Cell strainer (40 μm) | Labclinics | Cat# PLC93040 |
| Pasteur pipette | Labbox | Cat#PIPP-E03-1K0 |
| MS columns (optional) | Miltenyi Biotec | Cat#130-042-201 |
| LS columns (optional) | Miltenyi Biotec | Cat#130-042-401 |
| OctoMACSTM separator (optional) | Miltenyi Biotec | Cat#130-042-108 |
| MidiMACSTM separator (optional) | Thermo Fisher | Cat#130-042-302 |
| BD FACSAria IIIb sorter (optional) | Becton Dickinson | N/A |
a
LSR-Fortessa Cytometer: 355, 405, 488, 561 and 640 nm lasers
b
BD FACSAria III: 375, 405, 488, 561 and 640 nm lasers; 100 μm nozzle.