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. Author manuscript; available in PMC: 2021 Apr 20.
Published in final edited form as: Methods Cell Biol. 2020 May 13;159:1–22. doi: 10.1016/bs.mcb.2020.04.009

FIG. 4.

FIG. 4

Immunofluorescent analysis of HEOs and raft cultures. HEOs (Top panel) and organotypic raft cultures (Bottom panel) express esophagus epithelium specific markers. The entire epithelium is expressing the epithelium marker E-cadherin. Basal layers cells expressing P63 and SOX2 but lack the expression of keratin 13 (KRT13) and involucrin (IVL) as marked by white arrows. Suprabasal layers of differentiated cells express KRT13 and IVL but lack P63 expression. Scale bar—50 μm.

Images where modified from Trisno, S. L., Philo, K. E. D., McCracken, K. W., Catá, E. M., Ruiz-Torres, S., Rankin, S. A., et al. (2018). Esophageal organoids from human pluripotent stem cells delineate Sox2 functions during esophageal specification. Cell Stem Cell, 23(4), 501–515.e7. doi: 10.1016/j.stem.2018.08.008 (Fig. 4), pseudo colors and brightness were altered from original images for visualization purposes.