Fig. 5.

sCDP/DOX/miR-122 effectively induced cell apoptosis and reduced drug-resistance protein expression. (a) Cell apoptosis of HepG2 cells qualified by flow cytometry at 48 h after various treatments. Q1: dead cells; Q2: late apoptotic cells; Q3: live cells; Q4: early apoptotic cells. (b) Quantification of miR-122 level using qRT-PCR (n = 3). (c) Relative mRNA expression of MDR1, MRP and Bcl-w determined by qRT-PCR at 48 h after various treatments (n = 3). (d) Expression levels of P-gp, MRP, Bcl-w, p53 and cleaved caspase-3 measured by western blot analysis at 48 h after various treatments. *P < 0.05, **P < 0.01 and ***P < 0.001 were used as significant difference when comparing with the control groups or between these two groups.