Fig. 7.
In vivo therapeutic effects of sCDP/DOX/miR-122. (a) Scheme of tumor inoculation and systemic injection (i.v. via the tail vein) of saline, free DOX, sCDP/DOX and sCDP/DOX/miR-122 in HepG2 tumor-bearing nude mice. Dose: DOX of 2.0 mg kg−1 and miR-122 of 50.0 nmol kg−1. (b) Tumor growth curves of tumor-bearing mice after various treatments (n = 6). (c) Image tumors removed from the mice at the end point of the study. (d) Immunohistochemistry images of tumor sections stained by anti-Ki67 antibody and TUNEL assay. The brown color represents staining of positive cells. (e) HE stained images of various tissues from the mice. *P < 0.05 and **P < 0.01 were used as significant difference between these two groups.