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. 2021 Apr 2;27:161–178.

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Copyright © 2021 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Gene expression of NADPH oxidase enzyme. Relative mRNA expression of (A) NOX1, (B) NOX2, (C) NOX4, (D) p22phox, and (E) p47phox, in the eyes of normotensive (CONTROL) and hypertensive (Nω-nitro-L-arginine methyl ester, L-NAME) rats. The quantitative fold changes in gene expression were determined as relative to GAPDH in each corresponding group and calculated using the 2^-ΔΔCt formula. Values are expressed as mean ± standard error of the mean (SEM) of seven animals per group. **p<0.01, ***p<0.001 versus the CONTROL group.