Table 2. Antibodies used for western blotting analyses.
| Primary antibody | Origin | Dilution | Secondary Antibody | Dilution |
|---|---|---|---|---|
| Anti-NOX1 |
Mouse monoclonal |
1:1000 |
Goat anti-mouse |
1:2000 |
| Anti-NOX2 |
Rabbit monoclonal |
1:8000 |
Goat anti-rabbit |
1:4000 |
| Anti-NOX4 |
Rabbit polyclonal |
1:1000 |
Goat anti-rabbit |
1:2000 |
| Anti-p22phox |
Rabbit polyclonal |
1:1000 |
Goat anti-rabbit |
1:2000 |
| Anti-p47phox |
Rabbit polyclonal |
1:1000 |
Goat anti-rabbit |
1:2000 |
| Anti-eNOS |
Mouse monoclonal |
1:2000 |
Goat anti-mouse |
1:4000 |
| Anti-iNOS |
Mouse monoclonal |
1:2000 |
Goat anti-mouse |
1:4000 |
| Anti-nitrotyrosine |
Mouse monoclonal |
1:1000 |
Goat anti-mouse |
1:2000 |
| Anti-GSH-Px |
Rabbit polyclonal |
1:2000 |
Goat anti-rabbit |
1:4000 |
| Anti-GSH-Red |
Rabbit polyclonal |
1:5000 |
Goat anti-rabbit |
1:8000 |
| Anti-SOD |
Mouse monoclonal |
1:1000 |
Goat anti-mouse |
1:2000 |
| Anti-GFAP |
Mouse monoclonal |
1:2000 |
Goat anti-mouse |
1:4000 |
| Anti-GAPDH | Mouse monoclonal | 1:10000 | Goat anti-mouse | 1:20000 |