Figure 3.

Autophagy in LECs dampens CIA severity. (A–C) CIA was induced in Tx-treated Atg5^WT and Atg5^ΔProx1 mice. (A) Clinical scores (paw thickness) were assessed at indicated time points. Data are representative of two independent experiments with six to eight mice/group. (B) Inflammatory scores were assessed by histology analysis of knee sections at day 50 (six or seven mice/group). Representative images are provided, and arrowheads indicate inflammatory areas. Histograms represent global inflammation scores (sum of two knees per mouse). (C) mSAA and IL-6 mRNA levels in dLNs and paws at day 31 of CIA (four to six mice/group). (D) CIA was induced in Tx-treated Atg5^WT and Atg5^ΔFlt4 mice. Clinical scores (paw thickness) were assessed at indicated time points. Data are representative of two independent experiments with six to eight mice/group. (E) CIA was induced in Tx-treated Atg5^WT and Atg5^ΔProx1 mice. Flow cytometry analysis of indicated T cell subsets infiltrating hind-joint legs at day 31 (five or six mice/group). Representative dot plots are provided. Histograms represent the frequencies or the numbers of indicated cells in joints. Two-way ANOVA (A and D) and unpaired t test (B, C, and E). * P < 0.05, ** P < 0.01, and *** P < 0.001. Error bars correspond to lower and higher values for each group (B, C, and E) and to SEM (A and D).