Figure S3.

Abolition of MHCII in LECs impacts T reg cells during CIA. CIA was induced in Tx-treated MHCII^WT and MHCII^ΔProx1 mice, and T reg cells were analyzed in dLNs at days 26 and 31. (A and B) CD4⁺Foxp3⁺ cells (T reg cells) and T conv (CD4⁺Foxp3⁻) cell frequencies (A) and proliferation rate (Ki67⁺; B). (C and D) CD103 frequencies (C) and median (D) among T reg cells (CD4⁺FoxP3⁺; C) and CD103⁺ T reg cells (CD4⁺FoxP3⁺CD103⁺; D), CD25 negative (neg), or expressing low or high levels of CD25. Five or six mice/group, two independent experiments. Unpaired t test (A and B) and two-way ANOVA (C and D). * P < 0.05, ** P < 0.01, and *** P < 0.001. (A–D) Error bars correspond to lower and higher values for each group.