Figure S4.

Abolition of autophagy in LECs does not impair lymphatic vessel organization and draining functions upon inflammation. (A–C) Tx-treated Atg5^WT and Atg5^ΔProx1 mice were injected with OVA + CFA. 5 d later, lymphatic vessels (Lyve1⁺) were analyzed on sections from dLNs (A) and back skin (B). Images show organs from individual mice. (C) Dextran-FITC (40 kD) was injected in the left footpad of Atg5^WT and Atg5^ΔProx1 mice 5 d after OVA + CFA injection. 30 min later, dextran-FITC mean fluorescence intensity (MFI) was measured in draining (popliteal and inguinal) LNs to evaluate lymphatic vessel draining functions. Right footpads were used as ipsilateral controls to remove background signal. Data are representative of two independent experiments, four or five mice/group each. (C) Unpaired t test. Error bars correspond to lower and higher values for each group.