Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 15;218(6):e20202032. doi: 10.1084/jem.20202032

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Li et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Arrested NK cell maturation in Id2^Δ/Δ mice. (A) Flow cytometry showing gating strategy for BM NK cells in Ctrl and Id2^Δ/Δ mice. Top panels show lineage (Lin; CD3e)/PI versus NK1.1 on lymphoid cells in the BM. Lower panels show NK1.1 versus CD49b on Lin/PI-NK1.1⁺ cells. The percentage of cells in the gate is indicated. (B) Summary of the number of NK cells per 10⁸ BM cells. Bar represents the average ± SEM. Ctrl (white) and Id2^Δ/Δ (blue). Each circle represents one mouse. (C) Same as A but for spleen. (D) Same as B but for spleen. (E) CD49b⁺NK1.1⁺ NK cells from the BM (top) or spleen (bottom) were examined for expression of CD27 and CD11b by flow cytometry. The percentage of cells in each quadrant is indicated. (F and G) Summary of the number of CD27⁺CD11b⁻, CD27⁺CD11b⁺, and CD27⁻CD11b⁺ NK cells per 10⁸ BM cells or per spleen (n = 5 for BM and n = 3 for spleen [Sp]; data are from independent experiments). (H and I) RNA expression for the indicated genes in Ctrl or Id2^Δ/Δ determined by RNA-seq and expressed as normalized reads. Data are from three biological replicates. Error bars represent SD. Statistical significance was determined by two-tailed unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.005.