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. 2021 Apr 15;218(6):e20202032. doi: 10.1084/jem.20202032

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© 2021 Li et al.

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Figure 3. — Deletion of Tcf7 in mature NK cells impacts their numbers and maturation. (A) Flow cytometry for BM NK cells in Ctrl and Tcf7^Δ/Δ mice. Top panels show lineage (Lin)/PI versus NK1.1 on lymphoid cells. Bottom panels show NK1.1 versus CD49b on Lin/PI-NK1.1⁺ cells. The percentage of cells in the gated region is indicated. (B) Summary of the number of NK cells per 10⁸ BM cells in Ctrl (white) and Tcf7^Δ/Δ (maroon). Bars represent the average ± SEM. Each circle represents one mouse (n = 5). (C) Same as A but for spleen. (D) Same as B but for spleen (n = 4). (E) NK1.1⁺CD49b⁺ NK cells from BM (top) or spleen (Sp; bottom) were examined for expression of CD27 and CD11b by flow cytometry. Numbers indicate the percentage of cells in the respective quadrant. (F and G) Summary of the percentage of CD27⁺CD11b⁻, CD27⁺CD11b⁺, and CD27⁻CD11b⁺ NK cells among CD49b⁺NK1.1⁺ NK cells in BM and in spleen (n = 3; all flow cytometry data are from independent experiments). (H) RNA expression for the indicated genes in Ctrl or Tcf7^Δ/Δ NK cells determined by RNA-seq and expressed as normalized reads. Data represent three biological replicates. Error bars represent SD. Statistical significance was determined by two-tailed unpaired t test. *, P < 0.05; **, P < 0.01; ***, P < 0.005.