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. 2021 Apr 15;218(6):e20202032. doi: 10.1084/jem.20202032

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© 2021 Li et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure S1. — Alterations in gene expression in Id2^Δ/Δ BM NK cells. (A) CD49b⁺-enriched NK cells from BM were culture in 20 ng/ml IL-15 for 6 d, and then NK1.1⁺CD49b⁺ NK cells were analyzed for CD27 and CD11b by flow cytometry. Numbers indicate the percentage of cells in the respective quadrant (n = 4 from four independent experiments). (B) Summary of relative CD27 MFI. MFI of Ctrl NK cells was set as 1 in each experiment. Error bars represent SEM. Statistical significance was determined by two-tailed unpaired t test. ***, P < 0.005. (C) Volcano plot showing Log2FC versus adj. P value for RNA-seq data from Id2^Δ/Δ and Ctrl NK cells. Data are combined from three biological replicates. (D and E) Example of enrichment data from GSEA of Ctrl and Id2^Δ/Δ RNA-seq data. FDR, false discovery rate; NES, normalized enrichment score.