Viral production and cytopathogenicity of SARS-CoV-2 in Vero-E6 and six different cell lines of human origin
Indicated cell lines were infected with SARS-CoV-2 at moi of 1 and 0.1 either in duplicate or triplicate.
(A) Viral supernatant samples were harvested at 3hpi, 24hpi, 48hpi, 72hpi, 96hpi, and 120hpi. Viral production was determined by qRT-PCR targeting the N gene of SARS-CoV-2 comparing each time point with 3hpi.
(B) Cell viability was measured at 3 h post infection (hpi), 24hpi, 48hpi, 72hpi, 96hpi, and 120hpi by Viral-ToxGlo assay. The viability at each time was determined in comparison to the uninfected control.