Figure 2.

Cell-specific and strain-specific differences in SARS-CoV-2 tropism

(A) Scanning electron microscopy images of the cell surface of mock-infected and SARS-CoV-2-infected (moi 0.1) Caco2 and Calu-3 cells. Indicated cell lines were infected with SARS-CoV-2 at moi of 0.1 and fixed at 48hpi for microscopy analysis.

(B) ACE2 and TMPRSS2 receptor expression in six human cell lines. ACE2 and TMPRSS2 expression in the indicated cell lines was determined by western blot in the cell lysates. Heatmaps with relative protein quantification (blue), susceptibility (orange), and cytotoxicity (green) are shown.

(C) Susceptibility of globally isolated SARS-CoV-2 strains. Based on previously published or preprint articles, a chart was created showing the susceptibility of the commonly used laboratory cell lines and cytopathogenicity of SARS-CoV-2 strains isolated from different geographical locations. Boxes are color coded based on intensity as indicated in the figure. The moi used wherever available is mentioned in the Table S2.

(D) Table showing the presence of any amino acid substitution in the spike protein of the indicated strains. NA = not available.