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. 2021 Apr 20;24(5):102420. doi: 10.1016/j.isci.2021.102420

Figure 5.

Figure 5

Different expression of ISGs in the infected cell lines

(A) The gene expression level of IFN-β and indicated representative interferon-stimulated genes (ISGs): IFIT1, MX2, MX1, ISG15, and RIG-I in Calu-3, Caco2, 293FT, and Huh7 cell lines at 24hpi. The results are shown as fold change relative to mock-infected cells, normalized to GAPDH. The mean ± SEM of three experiments is shown. p values are determined by unpaired t test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. M = mock; V = virus.

(B) Protein expression levels of MDA-5, RIG-I, pIRF-3, p-STAT1, and viral nucleocapsid in SARS-CoV-2-infected and mock-infected Calu-3, Caco2, 293FT, and Huh7 cells at 24hpi.

(C) The intensity of specific bands for MDA-5, RIG-I, mono-ISG15, and SARS-CoV-2 nucleoprotein was quantified by ImageJ and the protein intensity normalised to β-actin is represented as bars. The intensity of p-IRF3 and p-STAT1 was not quantified, as expression could be observed only in one of the experimental replicates. The mean ± SEM of three experiments is shown.