Neutrophil gelatinase–associated lipocalin rescues cerulein-induced cell death in mouse acinar cells. A, MTT assay for evaluating cerulein dose response in pancreatic acinar cell viability during a 12-hour treatment. B, Viability of acinar cells in the presence of 20 μM cerulein along with 800 and 1600 ng/mL levels of rmNGAL for 12 hours as measured by MTT assay. C, D, Effect of rmNGAL in preventing cerulein-induced acinar cell death as demonstrated by annexin V/PI live dead staining. E, The rmNGAL blocks cerulein-induced acinar cell death by modulating PARP expression as demonstrated by Western blot analysis. *P < 0 0.05, **P < 0.01, ***P < 0.001, ns, not significant.