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(A) Workflow for label-free quantification of N-glycopeptides. (B) Standard haptoglobin (Hp) was separated by 1D gel. (C–E) Reproducibility analyses of four technical replicates performed at four different times. (C) The Pearson Correlation Coefficient R2 values in technical repetition. (D) The frequency of N-glycopeptide identification in technical repetition. (E) The reproducibility of glycopeptide intensity at N241 site in the four technical repeats.
