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. 2021 Apr 6;11:651421. doi: 10.3389/fonc.2021.651421

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Copyright © 2021 Cao, Cao, Shao, Liu, Yan, Meng, Zhang, Chen, Huang, Shu and Lu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Equal volumes of serum were acquired to separate 40-kDa band. (B) A total of 195 N-linked glycopeptides were identified, belonging to 33 kinds of glycan compositions. (C) Based on four biological repeats (each pooled from five randomly selected individuals), 26 N-linked glycopeptides appeared more than three times (QC2) and passed strict screening criteria (QC3) were considered. (D) Three glycopeptides corresponding to APOH, PON1, and HPTR/HPT were changed significantly in AFP-negative HCC compared with LC patients.