Purification of xyloglucanases from V. dahiae.

The reactions were carried out in 40 mM phosphate buffer, pH 7.0, at 40 °C with 0.4 % xyloglucan as the substrate.

Purification step (mg)	Protein	Total activity (U)	Specific activity (U/mg)	Yield (%)	Purification (fold)
Crude	595.9	220.6	0.37	100	1.0
Ammonium sulfate　precipitation	72.7	67.9	0.93	30.8	2.5
Butyl Toyopearl 650M
Xgl12A	7.1	33.2	4.70	15.0	12.7
Xgl74B	4.9	16.9	3.42	7.7	9.2

The reactions were carried out in 40 mM phosphate buffer, pH 7.0, at 40°C with 0.4% xyloglucan as the substrate.