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. 2017 Sep 20;64(4):83–90. doi: 10.5458/jag.jag.JAG-2017_005

Fig. 1. DEAE-Sephacel and Sepharose CL-4B column chromatography of hot water extract of salmon cartilage.

Fig. 1.

　Hot water extract of salmon cartilage was fractionated by DEAE-Sephacel chromatography (A) followed by Sepharose CL-4B chromatography (B). Broken lines indicate the gradient curves of NaCl and arrows the specific NaCl concentrations. Solid circles, uronic acid content detected by carbazole sulfuric acid method; open circles, protein content detected by the method of Bradford (A) or protein content by monitoring the UV absorbance at 280 nm (B). Bars with Roman numerals indicate the fractions combined for recovery of material as: pool I (Frs. 154–176), pool II (Frs. 25–31), pool III (Frs. 37–41), pool IV (Frs. 53–57), and pool X (Frs. 112–132). Pool I from DEAE column chromatography was further fractionated by Sepharose CL-4B column chromatography and the resulting pools II, III, and IV were recovered and analyzed. Pool X was collected for the analysis of HA.