Fig. 2. Gel filtration HPLC of PGs purified from hot water extract of salmon cartilage.

　PGs in pools II, III, and IV (Fig. 1), and PG purified from 4 M GdnHCl-extract were analyzed by Shodex OHpak SB-805 HQ gel filtration column before treatment (a) or after treatment with LiOH followed by chondroitin ABC lyase (b) or after treatment with actinase E (c). Solid arrows indicate the elution positions of size standards of pullulan with defined approximate molecular weight. Dashed arrows indicate the void volume (V₀) and total column volume (V_t). II-1 and II-2 indicate the peak and the shoulder, respectively.