. 2017 Sep 20;64(4):83–90. doi: 10.5458/jag.jag.JAG-2017_005

Table 1.

Molecular size estimation of PGs purified from hot water extract (A, B, C) and the number of repeating disaccharide units (D) and the number of GAG chains calculated from them (E).

Calculated molecular weight	PG from hot water extract			PG from 4 M GdnHCl-extract^c
Calculated molecular weight	Pool II	Pool III	Pool IV	PG from 4 M GdnHCl-extract^c
(A) Non-treated	700,000–8,600,000 II-1 (4,100,000)^d II-2 (1,520,000)	35,000–7,500,000 (810,000*)	1,000–533,000 (138,000*)	85,000–5,300,000 (1,100,000*)
(B) Deglycosylated^a 　　(core protein)	2,750–540,000 (117,000*)	2,700–650,000 (100,000*)	N.D.–147,000 (23,700*)	3,750–2,000,000 (123,000*)
(C) Peptide-degraded^b 　　(GAG chain)	N.D.–112,000 (23,100*)	N.D.–130,000 (23,100*)	160–142,000 (23,500*)	50–159,000 (20,000*)

(D) Number of repeating disaccharide units	N.D.–280.2 (56.5**)	N.D.–325.5 (56.5**)	N.D.–355.7 (57.5**)	N.D.–398.5 (48.7**)
(E) Number of GAG chains	72.0–N.D. II-1 (172.4) 72.0–N.D. II-2 (60.7)	52.7–N.D. (30.7**)	2.7–N.D. (4.9**)	20.8–1562.5 (48.9**)

PG samples were analyzed by gel filtration HPLC using a Shodex OHpak SB-805 HQ column. Various sizes of pullulan were used as size standards. PG, proteoglycan. Pools II, III, and IV were obtained in CL-4B chromatography (see Fig. 1). ^aDeglycosylation by LiOH treatment followed by chondroitin ABC lyase digestion. ^bPeptide-degradation by treatment with Actinase E. ^cThe same sample as the one used in Carbohydr. Polym., 103, 538–549 (2014).⁵⁾ ^dII-1 and II-2 are indicated in Fig. 2. *Molecular sizes of material eluting at the peak position and **the values calculated from them.