Figure 2.

The effect of disease-associated TRPM3 mutations on agonist sensitivity and heat sensitivity

Adapted from Zhao et al. 2020 [37]. A-C: Representative two electrode voltage clamp experiments in Xenopus oocytes expressing the hTRPM3₁₃₂₅ splice variant (A) and its mutants V990M (B) and P1090Q (C). Horizontal lines show the applications of different concentrations of PregS (μM). D: Hill fits of the concentration dependence of the effect of PregS. E-G: Representative whole cell patch clamp measurements on the human TRPM3α2 (A) or its mutants V992M (B) and P1092Q (C) expressed in HEK293 cells. Note that the numbering of the residues differs by 2 because of the different splice variant used compared to (A-C). The applications of 100 μM PregS are indicated by the horizontal lines, the temperature curves on the top panels. D: Summary of the slopes of the current increases between 23°C and 33°C. See Zhao et al. 2020 [37] for more details.