Table 2.

List of initial workup studies for patients presenting with a possible diagnosis of pure red cell aplasia

Studies
Anemia
CBC with differential
Reticulocyte count
EPO levels
Coombs test
Nutritional deficiencies
Hb electrophoresis and peripheral blood smear examination
Diamond-Blackfan anemia
Erythrocyte ADA (before transfusion of red cells)
Genetic testing
LGL
Flow cytometry (as part of a low-grade lymphoma panel)
TCR rearrangement
STAT3 gene mutations (eg, in order of frequency Y640F, D661Y, D661V, N647I)
Autoimmunity and immunologic conditions
Antinuclear antibodies
Rheumatoid arthritis antibodies
Extractable nuclear antigen panel
Lymphocyte subsets (flow cytometry)
B-cell dyscrasias studies
Serum electrophoresis
Serum immunofixation
Free light chains
Immunoglobulins levels
Bone marrow
Morphology (myeloid:erythroid ratio, dysplastic changes, giant pronormoblasts)
Flow cytometry for LGL
Cytogenetics
TCR rearrangement
Virological
B19 parvovirus
Viral hepatitis
HIV
EBV
CMV
Anamnestic data about drug history
Thymoma studies*
CT scan of the chest

^*Particulary in case of history of myasthenia gravis.

ADA, adenosine deaminase; EBV: Epstein-Barr virus; EPO, erythropoietin; STAT3, signal transducer and activator of transcription 3.