Figure 2. CPAP depletion enhances the tumorigenic properties of OSCC cell lines.
SCC-Cal27 and UM-SCC-74B cell lines were transduced with control-shRNA or CPAP-shRNA (under doxy-inducible promoter) lentiviral particles, and the cells with stable integration were selected using puromycin. (A) shRNA expression was induced in these cells by culturing for 72 h in the presence of doxy (10 μg/ml) and subjected to IB to detect CPAP and β-actin. (B) Eight-week-old nude mice were injected s.c. with these control-shRNA and CPAP-shRNA lentivirus transduced cells (approx: 2 × 106 cell/mouse) on the flank, after mixing with equal volume of matrigel, and monitored for tumor progression. These mice were euthanized after 24 days (UM-SCC-74B cell recipients) or 48 days (SCC-Cal27 cell recipients) to determine the tumor weight. Cumulative data from two independent experiments (total of 11 mice/group for SCC-Cal27 cells and 14 mice/group for UM-SCC-74B cell recipients) are shown (B; upper panels). Images of tumors harvested from one experiment (7 mice/group) are also shown (B; lower panels). P-values are by two-tailed, unpaired Mann-Whitney test.