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. 2021 Apr 13;12(8):807–822. doi: 10.18632/oncotarget.27932

Figure 4. EGFR depletion diminishes the EMT phenotype and invasive property of CPAP-depleted OSCC cells.

Figure 4

Control-shRNA and CPAP-shRNA expressing indicated cells were treated with control-siRNA or EGFR-siRNA for 48 h. (A) Cells were subjected to IB to detect EMT associated transcription factors Slug and Zeb along with EGFR and β-actin. Equal number of each cell types were also subjected to migration and matrigel-invasion assay using the transwell approach and imaged. (B) Representative fields of migrated cells on insert membranes with no matrigel coating are shown. (C) Representative fields of invasive cells on membranes with matrigel coating (upper panels) and average number (mean ± SD) of cells of at least 5 fields/group (lower panels) are shown. Representative results from one of the three independent experiments are shown. P-values are by two-tailed, unpaired Mann-Whitney test.