Table 1. Samples and variant types utilized in the analytic validation.

Variants Validated	Validation Samples	Source of Validation	# of Variants for Validation
SNV and Indels	73 clinical samples, 4 reference standards	Tumor DNA	517 SNV events, 30 indel events
Copy Number Alterations	88 clinical samples, 2 reference standards	Tumor DNA	43 CNA events
Gene Fusions	32 clinical samples (FFPE), 2 cell lines, 4 commercial reference standards	Tumor RNA	91 fusion or special transcript events
MSI	29 patient samples orthogonally tested for MSI Status	Tumor DNA	Exome Wide
TMB	23 patient samples orthogonally tested for TMB status	Tumor DNA	Reported as low, intermediate, or high