Skip to main content
. 2021 Apr 20;14(4):963–972. doi: 10.1038/s41385-021-00405-7

Fig. 2. DP1 agonism-induced mortality is associated with exaggerated lung inflammation.

Fig. 2

a–e WT BALB/c mice were intranasally inoculated with PVM at PND 7 (0 dpi) and treated daily with DP1 agonist (BW245c) or vehicle from 3 to 6 dpi (intranasal). Mice were euthanized at 5, 7, and 10 dpi. a Concentrations of IFN-λ and IFN-γ in lung homogenates and BALF, respectively. b Representative micrographs (x20) of PVM immunoreactivity in the lung airway and parenchyma (scale bar = 100 µm) and quantification of PVM-positive cells in the lung parenchyma. Arrows indicate the presence of dead cells. c Concentration of dsDNA in BALF. d Representative micrographs (x20) of haematoxylin and eosin stained-lung tissue (scale bar = 100 µm) and lung inflammation score. e Concentrations of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 in the lung homogenates and sIL-6R in the BALF. f Level of PGD2 in the lung homogenate. Data are presented as box-and-whisker plots with boxes representing quartiles and whiskers indicating the range and are pooled data from two independent experiments (n = 5–12 mice per group). Two-way ANOVA with Bonferroni post hoc test was used for comparisons (*p < 0.05, **p < 0.01, ***p < 0.001).