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. 2021 Apr 19;47(6):109. doi: 10.3892/ijmm.2021.4942

Figure 2.

Figure 2

Overexpression of NFKBIZ affects bladder cancer cell proliferation, migration and invasion in vitro. (A) Representative images of transfected T24 and 5637 cells were captured using a fluorescence microscope (magnification, ×200). NFKBIZ transfection efficiency was evaluated by (B) Reverse transcription-quantitative PCR and (C and D) western blotting in T24 and 5637 cell lines. Representative images and quantitative analyses of (E and F) T24 and (G and H) 5637 cell colonies in each group according to colony formation assays. Representative images and quantitative analyses of (I and J) T24 and (K and L) 5637 cell migration rates in each group according to wound healing assays (magnification, ×200). Representative images and quantitative analyses of invasive (M and N) T24 and (O and P) 5637 cells in each group according to Transwell invasion assays (magnification, ×200). **P<0.01 and ***P<001 vs. NC. NC, negative control; NFKBIZ, NF-κB inhibitor ζ.