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. 2021 Mar 4;41(5):1675–1693. doi: 10.1161/ATVBAHA.120.315525

Figure 7.

Figure 7.

Circ-Calm4 small-interfering RNA inhibits pyroptosis in pulmonary arterial smooth muscle cells (PASMCs) through the miR-124-3p/PDCD6 (programmed cell death protein 6) pathway. A and B, Knockdown of endogenous circ-Calm4 abrogated upregulation of PDCD6 protein and Pdcd6 mRNA expression induced by hypoxia (n=6). C and D, MiR-124-3p reversed but AMO-124-3p enhanced the upregulation of PDCD6 protein and Pdcd6 mRNA expression induced by hypoxia (n=6). E and F, In PASMCs, knockdown of circ-Calm4 reduced PDCD6 protein and Pdcd6 mRNA expression induced by hypoxia, whereas AMO-124-3p reversed the downregulation of PDCD6 protein and Pdcd6 mRNA expression (n=6). G and H, Knockdown of circ-Calm4 by sh-circ-Calm4 countered the hypoxia-induced upregulation of PDCD6 at the protein and mRNA levels (n=6). I, Circ-Calm4 expression was positively correlated with Pdcd6 mRNA expression in hypoxic mice. In hypoxic mice, circ-Calm4 expression was negatively correlated with miR-124-3p expression, and Pdcd6 mRNA expression was negatively correlated with miR-124-3p expression. Each datapoint in the figure represents a unique biological replicate. The data are presented as the means±SD. Statistical analysis was performed with 1-way ANOVA followed by Bonferroni correction. *P<0.05, **P<0.01, ***P<0.001.