Fig. 3.

Splenic function was tied to IL-35 in a CLP-induced ARDS model. C57BL/5 mice (5/group) were subjected to LPS, LPS (splenectomy), CLP or CLP (splenectomy). The sample was extracted and detected by referring to the aforementioned method. IL-35 in serum, BALF, and lung homogenates in the splenectomized CLP group were significantly lower than those in the nonsplenectomized CLP group. In the LPS and splenectomized LPS groups IL-35 in serum, BALF, and lung homogenate did not differ significantly. **p < 0.01, and ****p < 0.0001, by the two-way ANOVA followed by LSD multiple comparisons test, compared with the no-splenectomy group.