Fig. 4.

ARDS was induced in wild-type mice via CLP or LPS. T cells were isolated from mice spleen and the frequency of Tregs was subsequently determined by flow cytometry, five mice per time point. A and B were representative dot plots as shown. (A–D) Treg cells gradually decrease with the prolongation of time in CLP-induced ARDS, but not in LPS. (E–F) Ratio of CD4+/Treg in spleen was significantly increased in CLP-induced ARDS, but not in LPS. Ratio of CD4+/CD4+CD25+ and CD4+CD25+/Treg in spleen showed no significant change in the two ARDS models. *p < 0.05 ****p < 0.0001, by two- way ANOVA followed by LSD multiple comparisons test, compared with the LPS + IgG group.