Table 3.
Antibodies used for immunohistochemistry. Abbreviations: APP = amyloid precursor protein; GAP43 = growth‐associated protein 43; H = high molecular weight; MW = microwave pretreatment; NF = neurofilament; nonP = nonphosphorylated; P = phosphorylated; pc = polyclonal.
| Antigen | Clone | Dilution | Pretreat‐ment | Source |
|---|---|---|---|---|
| MBP | Rabbit pc | 1:500 | MW | DakoCytomation, Glostrup, Denmark |
| CNPase | SMI91 | 1:200 | MW | Covance Inc., Princeton, NJ, USA |
| Macrophages/microglia | KiM1P | 1:5000 | MW | (32) |
| β‐tubulin | TUB 2.1 | 1:400 | MW | Sigma‐Aldrich Inc., Saint Louis, MI, USA |
| GAP‐43 | 9‐1E12 | 1:4000 | MW | Chemicon International, Temecula, CA, USA |
| Synaptophysin | SY38 | 1:10 | MW | DakoCytomation |
| APP | 22C11 | 1:3000 | MW | Chemicon International |
| nonP‐NF‐H | SMI32 | 1:7000 | MW | Covance Inc. |
| P‐NF‐H | SMI31 | 1:1000 | MW | Covance Inc. |