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. 2011 Nov 8;22(4):485–498. doi: 10.1111/j.1750-3639.2011.00540.x

Figure 3.

Figure 3

Localization of phenylalanyl‐tRNA synthetase alpha chain (FARSA) and gammabutyrobetaine dioxygenase (BBOX1) by immunohistochemistry in paraffin sections (10 µm) of the locus ceruleus of PD patients, controls and AD patients. Alpha‐synuclein and Nissl staining of the locus ceruleus (LC) of controls (A,D), PD patients (G,J) and AD patients (M,P) revealed neuronal loss in PD and AD patients and alpha‐synuclein immunostained Lewy bodies and Lewy neurites in PD patients. BBOX1 was detected in both soma and cellular processes of glial cells of the LC of controls (B,E), PD patients (H,K) and AD patients (N,Q); arrows in picture H indicate BBOX1 staining surrounding LC neurons in a PD patient with relatively little cell death; enriched staining pattern in blood vessel endothelium is pointed out by arrow in picture K. In controls (C,F), PD (I,L) and AD patients (O,R) FARSA staining in the LC was localized in the cytoplasm and cellular processes of both neuronal and glial cells. In the cytoplasm of neurons, FARSA staining was diffuse and granular, predominantly near the cell borders and the nuclear membrane; arrows denote FARSA staining in a number of glial and neuronal processes. Scale bar = 1 µm.