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. 2021 Apr 16;14:1511–1535. doi: 10.2147/JIR.S300025

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© 2021 Alrumaihi et al.

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Effect of FSE on p53 signaling using the Western blotting.

Notes: (A) MCF-7 and (B) SK-BR3 cells (1 × 10⁶) were treated with indicated concentrations of FSE for 48 hours in 100 mm plates. The cells were lysed to isolate the whole-cell protein for immunoblotting using antibodies specific for p53, p21, Bax, Bcl-2, and β-actin. The data are shown as the mean ± SEM of triplicate experiments. Statistical differences were analyzed by Two-way ANOVA, Tukey’s multiple comparison test. *Significant difference vs FSE 0, 40, and 80, ^#significant difference between each treated group.