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. 2020 Sep 1;28(3-4):321–334. doi: 10.1038/s41417-020-00221-4

Fig. 2. Vector schematics and LV CAR T-cell transduction efficiencies.

Fig. 2

a Schematic diagrams showing the EphA2 (T-EphA2) and ΔCAR (T-Δ) constructs used to generate LV vectors for transduction of T cells. For each A2 construct (containing either the tCD19 OR tEGFR sequence downstream of the CAR sequence) an equivalent control construct was used to generate Δ CAR T cells in all experiments. b Representative flow cytometry dotplots showing dual CD3 (FITC, Y axis) and tCD19 (PE, X axis) labelling of control nontransduced (T-NT) T cells, ΔCAR (T-∆) or EphA2 CAR (T-EphA2) T cells generated from the same donor. c Representative flow cytometry dotplots showing tEGFR (Alexa Fluor 488, X axis) against Forward Scatter (Y axis) labelling of control nontransduced (T-NT) T cells, ΔCAR (T-∆) or EphA2 CAR (T-EphA2) T cells generated from the same donor.