Fig. 3.

Yield and enrichment of 80- and 641-amino acid linkers in a CD276 system. Yeast displaying either pCT-80 or pCT-641 tethered ligands were mixed with nondisplaying yeast and sorted in parallel by adherent cell panning. (A) Enrichment ratio, (B) ligand-displaying yeast yield and (C) nondisplaying yeast yield were quantified. Ligand: AC2. Cell Line: MDA-MB-231. The dotted line (A) indicates the limit of functional enrichment. ^*P < 0.05 relative to the 80- amino acid linker