Fig. 1. TAZ expression is gradually increased during chondrogenic differentiation.

a Heatmap analysis of TAZ-associated genes in early murine limb (from E9.5 to E13.5; GSE30138³²). b Representative immunofluorescence-stained images of tibiae from WT mice at the ages of E16.5 and P1, labeled for TAZ and stained for DAPI. Scale bars, 75 μm. c qRT-PCR analysis of the chondrocyte differentiation markers of micromass culture as indicated time. d Whole-cell lysates from micromass culture were immunoblotted with antibodies against TAZ, Runx2, and GAPDH. e qRT-PCR analysis of TAZ expression using the articular cartilage from the WT mice at 1, 2, and 4 months. The experiment was independently repeated for three times. Error bars were the means ± SEM of triplicates from a representative experiment. *P < 0.05, **P < 0.01, ***P < 0.001.