Fig. 6. Deletion of TAZ blocks chondrocyte maturation via inhibiting Col10a1 expression.
a Micromass culture with primary chondrocytes from Col2-Cre;TAZf/f mice and controls. After cultures of 7 days, the macromass cultured chondroctyes were stained with Alcian blue as shown. b qRT-PCR analysis of Col10a1 in primary chondrocytes of Col2-Cre;TAZf/f mice and controls. c, d qRT-PCR analysis from silenced- and overexpressed-TAZ transduced chondrocytes and controls. e Immunofluorescence staining analysis for Runx2 expression in chondrocytes from Col2-Cre;TAZf/f mice and controls. Scale bars, 25 μm. f Whole chondrocyte lysates from Col2-Cre;TAZf/f mice and controls were immunoblotted with antibodies against Runx2, Col10a1, and GAPDH, respectively. g, h Col10a1 expression in chondrocytes after manipulation with Runx2, shTAZ, or TAZ as shown. i Schematic diagram of TAZ/Runx2-binding sites in the Col10a1 promoter (BS binding site; TSS, transcription start site). j ChIP assay. Co-occupation of TAZ/Runx2 in the Col10a1 promoter. The experiment was independently repeated for three times. Error bars were the means ± SEM of triplicates from a representative experiment. *P < 0.05, **P < 0.01.