Fig. 4. Discovery of a snoRNA–rRNA interaction network in rRNA processing and LCC.

a Intermolecular interactions among U8, U13, 18S, and 28S based on PARIS2 data from human HEK and mouse brain. Two intramolecular U8 alternative conformations are labeled in red and blue colors. Each row illustrates interactions between one pair of RNAs. Each column illustrates regions in one RNA that participate in interactions. b Mapping LCC mutations onto the U8 interaction network. Stemloops (SL) are defined for all U8 conformations and their interactions. U8d U8 dimer. All mutations from LCC patients were summarized in the track, where the coverage [0–7] indicates counts of mutations. c MFE changes for all U8 related duplexes due to LCC-causing mutations, calculated using PARIS2-derived models and RNAcofold. Red: that mutations increased the MFE and lowered stability of the duplex.