Fig. 5. RAC1B interacts with multiple membrane-bound receptor tyrosine kinases.

A Schematic of BioID protocol. B Venn diagram outlining RAC1 and RAC1B interacting proteins identified by MS and the overlap between the interactomes. C Network analysis of the RAC1/RAC1B interactome. D CMT93 cells were treated with NT or Rac1b PMO, serum-starved and then stimulated with EGF for 5, 10, 15 or 30 min before analysis by western blotting for EGFR^pY1068, EGFR and Vinculin (sample control blot). Representative blots shown. E EGFR^pY1068 band densitometry relative to EGFR (normalised to Vinculin) of 4 replicate time course experiments (data are presented as mean ± SEM; ^**P = 0.002; two-way ANOVA with Tukey multiple correction; n = 4v4). Source data are provided as a Source Data file.