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. 2021 Apr 20;12:2330. doi: 10.1038/s41467-021-22431-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–c Left: representative micrographs showing immunochemistry GFAP staining in the hypothalamus of RCD-fed GFP^Sf1 mice (GFP^Sf1-RCD) (a), HFD-fed GFP^Sf1 mice (GFP^Sf1-HFD) (b), and HFD-fed cPLA2KD^Sf1 (cPLA2KD^Sf1-HFD) mice (c). Scale bar: 500 μm. Right: magnified areas in the VMH and ARC in the left. Scale bar: 30 μm. d,e, Quantification of GFAP-positive cells in the VMH (d) or ARC (e) of GFP^Sf1-RCD (n = 4), GFP^Sf1-HFD (n = 4) and cPLA2KD^Sf1-HFD (n = 4) mice (one-way ANOVA followed by Sidak multiple comparison test, in e, p = 0.0007 GFP^Sf1 RCD vs GFP^Sf1 HFD, p = 0.0045 GFP^Sf1 HFD vs cPLA2KD^Sf1 HFD). f,g, Size of GFAP-positive cells in in the VMH (f) or ARC (g) of GFP^Sf1-RCD (n = 3), GFP^Sf1-HFD (n = 3), and cPLA2KD^Sf1-HFD (n = 3) mice (one-way ANOVA followed by Sidak multiple comparison test, in g, p = 0.0002 GFP^Sf1 RCD vs GFP^Sf1 HFD, p = 0.0012 GFP^Sf1 HFD vs cPLA2KD^Sf1 HFD). h–j Left: Representative micrographs showing immunochemistry Iba1 staining in the hypothalamus of GFP^Sf1-RCD (h), GFP^Sf1-HFD (i) and cPLA2KD^Sf1-HFD mice (j). Scale bar: 500 μm. Right: magnified areas in the VMH and ARC from the left photos. Scale bar: 30 μm. k, l Quantification of Iba1-positive cells in the VMH (k) or ARC (l) of GFP^Sf1-RCD (n = 3), GFP^Sf1-HFD (n = 3), and cPLA2KD^Sf1-HFD (n = 3) mice (one-way ANOVA followed by Sidak multiple comparison test, in l, p = 0.0049 GFP^Sf1 RCD vs GFP^Sf1 HFD, p = 0.0310 GFP^Sf1 HFD vs cPLA2KD^Sf1 HFD). m, n Size of Iba1-positive cells in the VMH (m) or ARC (n) of GFP^Sf1-RCD (n = 3), GFP^Sf1-HFD (n = 3), and cPLA2KD^Sf1-HFD (n = 3) mice (one-way ANOVA followed by Sidak multiple comparison test, in m, p < 0.0001 GFP^Sf1 RCD vs GFP^Sf1 HFD, p = 0.0004 GFP^Sf1 HFD vs cPLA2KD^Sf1 HFD, in n, p < 00001 GFP^Sf1 RCD vs GFP^Sf1 HFD, p = 0.0004 GFP^Sf1 HFD vs cPLA2KD^Sf1). All data represent the mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001.