Comparison of phylogenies based on nuclear genome and kinetoplast maxicircle sequences. (A) Whole-genome alignment confirmed the clustering of strains according to discrete typing unit (DTU), and particularly resolve the closely related TcV and TcVI DTUs. (B) The corresponding ML phylogenetic tree of the kinetoplast maxicircle resulted in the previously identified three A, B, and C clades. However, there was also a clear substructuring of the maxicircles from Clade B according to the DTUs TcIII, TcV, and TcVI. Numbers on branches indicate bootstrap support.