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. 2021 Apr 7;12:649572. doi: 10.3389/fimmu.2021.649572

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Copyright © 2021 Yashiro, Yamamoto, Araumi, Hara, Yogo, Uchida, Kasakura and Nishiyama

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of transcription factors that bind to the cis-elements. (A) Sequences of the probes used in electrophoretic mobility shift assay. (B, D) Fluorescein (FLO)-labeled probe A (B) or B (D) were incubated with nuclear extracts of THP-1 cells in the presence of anti-PU.1 (P), anti-IRF4 (4), anti-IRF8 (8) or nonspecific (N) antibodies. (C, E) FLO-labeled probe A (C) or B (E) were incubated with nuclear extracts of THP-1 cells in the presence of 2- or 10-fold amounts of unlabeled wild-type or mutated oligonucleotides. After electrophoresis on 5% acrylamide gels, fluorescence was detected.