Figure 4.

cSiO₂-induced inflammatory cell recruitment into the lungs is modestly attenuated by DHA feeding. 13 week following the final cSiO₂ instillation, mice were necropsied and BALF collected. The BALF cell fraction was assessed for (A) total cells, (B) macrophages, (C) neutrophils, and (D) lymphocytes as measures of inflammatory cell recruitment. cSiO₂ significantly increased the number of each cell type in the BALF. Total leukocytes, macrophages, and lymphocytes appeared to be slightly reduced by the high DHA diet, though the difference was not significant. Statistical comparisons were performed as described in the Methods section. ****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, ns = not significant.