Figure 7.

High DHA diet reduces CD45R⁺ B-cell infiltration in the lungs of cSiO₂-treated mice. Light photomicrographs of lung tissue sections immunohistochemically stained for CD45R⁺ B cells (arrows) from mice fed control diet and intranasally instilled with saline vehicle (Veh/CON, A), fed control diet and intranasally instilled with cSiO₂ (cSiO₂/CON, B), fed 0.4 % DHA diet starting one week after the end of the intranasal cSiO₂ instillations (cSiO₂/low DHA, C), or fed the 1.0% DHA diet starting one week after the end of the intranasal cSiO₂ instillations (cSiO₂/high DHA, D). Marked perivascular and peribronchiolar B lymphoid cell accumulations (arrows) in lung of cSiO₂/CON mouse. Moderate, minimal and no accumulations of B lymphoid cells around blood vessels (v) and bronchioles (b) in cSiO₂/low DHA (C), cSiO₂/high DHA (D), and Veh/CON (A) mice, respectively. a, alveolar parenchyma. (E) depicts percentage of CD45R+ stained lung tissue. Statistical comparisons were performed between groups, as described in the Methods section. ***p ≤ 0.001, *p ≤ 0.05, ns = not significant.