Fig. 6.

Time course of SYN and SWP after the soluble tumor necrosis factor receptor 1 (TNFR1) treatment (3 ng/µL; 60 ng/µL) in cells from mice lacking both TNFRs (TNFRKO) and cells from mice lacking TNF (TNFKO) on day 14. For each strain, sTNFR1 treatment dose, and parameter, graphically depicted values are means ± standard errors (SEs) of changes in synchrony (SYN) and slow wave delta power (SWP) every five minutes over the course of one hour. Numbers of preparations, MEAs, wells, and electrodes are presented in Table 1. A. Time course of SYN changes after treatment with either 3 ng/µL or 60 ng/µL sTNFR1 in TNFKO and TNFRKO cells. B. Time course of SWP (µV ², 0.25–3.75 Hz) changes after treatment with either 3 ng/µL or 60 ng/µL sTNFR1 in TNFKO and TNFRKO cells. Changes in each measure were compared first to their day 14 baseline values and then to the sTNFR1 control treatment change from baseline mean for each 5 min time period individually within the same MEA and strain as described in the methods section. Significance of time-induced changes (p < 0.05) was determined by one-way ANOVA (time) followed by post-hoc Tukey’s HSD tests when significant main effects were found within each strain, dose, and parameter. Significant effects of time, strain and time by strain interactions were determined by two-way ANOVA (time, strain, and time × strain) and, where indicated, post-hoc Tukey’s HSD tests within each dose for each parameter. Significant strain differences were found for 60 ng/µL dose only: TNFRKO vs TNFKO: ^{^^}p < 0.01. Within strain and dose, significant time differences vs 5 min: TNFRKO 3 ng, *p < 0.05; TNFKO 3 ng, $ $p < 0.01; TNFKO 60 ng, &p < 0.05, &&p < 0.01. Within strain and dose, significant time differences are reported in the results section.