Fig. 6. IL-10 deficiency increases monocyte-derived macrophage infiltration and changes its surface marker expression in vivo.

Male C57BL/6 (WT) and IL-10^−/− mice underwent collagenase injection or sham procedure. Brains were perfused with PBS and dissociated into single cells that were stained with antibodies for flow cytometry. (A) Representative flow plots show the CD11b⁺CD45⁺ population after gating the PI⁻ cells (population inside the solid line). Ma, macrophage; Mi, microglia. (B) Percentages of CD11b⁺ cells. *p < 0.05 vs. corresponding Sham; N.S., not significant; n = 3–4. (C) Percentages of CD45^highCD11b⁺/CD11b⁺ cells (macrophage) and CD45^intCD11b⁺/CD11b⁺ cells (microglia). *p < 0.05, **p < 0.01 vs. corresponding WT group; n = 3–4. (D-I) Cells were stained with different antibodies for flow cytometry. The absolute cell counts of CD86⁺CD11b⁺ (E) and CD206⁺CD11b⁺ (G) were quantified based on the total events. The percentages of CD86⁺CD11b⁺ (D, H) and CD206⁺CD11b⁺ (F, I) cells were quantified. Representative flow plots show CD45⁺CD86⁺ (D) and CD45⁺CD206⁺ (F) populations after gating the CD11b⁺PI⁻ cells. (E, G): *p < 0.05 vs. corresponding Sham; N.S., not significant. (H, I): *p < 0.05 vs. corresponding WT. n = 3–4. Results are presented as scatter plots (mean ± SD). B, E, G: One-way ANOVA followed by Dunn’s multiple comparison post-hoc test; C, H, I: Two-way ANOVA followed by Bonferroni post-hoc tests. All results are from at least three independent experiments. M/MФ: microglia/macrophages.